Category Archives: Protocols

DNAse-free RNA isolation (for qRT), the Citrate-Citric Acid method

introduction plant RNA isolations often requires the of use unhealthy organic solutions like phenol or beta-mercaptoethanol, and although I still prefer the TriZOL method or the SDS-TriZOL combo for when I need large quantities of great RNA, you have to wonder if it … Continue reading

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SDS-TRIzol Combo RNA isolation from high carbohydrate containing tissues

Introduction For simple tissues, I prefer to isolate RNA with the Citrate-Citric Acid method, but the isolation of good quality RNA of fruits and other plant tissue with a lot of sugars and/or other inhibiting crap in it can be … Continue reading

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RNA isolation with homemade TRIzol reagent

Introduction My all time favorite RNA isolation, and most used protocol is the TRIzol method. Here I give you the basic protocol, including the recipe to make it yourself, which will save you a lot of money. Method Grind ~100 … Continue reading

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